signal detection Search Results


sumoylation 2 3 affinity beads  (Cytoskeleton Inc)
93
Cytoskeleton Inc sumoylation 2 3 affinity beads
Fig. 5 ISG15 upregulated by PAPR12, increasing ISGylation of MFN1/2 and then attenuating the ubiquitylation and <t>SUMOylation</t> to inhibit PINK1/Parkin-dependent mitophagy. a Immunoblotting (IB) analysis of ubiquitylation and ISG15 in PHCs following transfection with KD-ISG15 or KD-NC. b Protein quantification of (a) using ImageJ. n = 3 per group. c IB analysis of ubiquitylation of mitochondrial proteins. Mitochondria were isolated from KD-NC and KD-ISG15 PHCs transfected with a HA-ubiquitin construct. d Protein quantification of (d) using ImageJ. n = 3 per group. e IB analysis of SUMO2/3 and ubiquitin with SUMO2/3-conjugated proteins in KD-NC and KD-ISG15 PHCs immunoprecipitated using SUMO2/3 affinity beads. f IB analysis of MFN1/2, MFN1, MFN2, ubiquitin, SUMO2/3 and ISG15 in KD-NC and KD-ISG15 PHCs treated with or without MG-132 after immunoprecipitation of MFN1/2. The molecular marker of IB plots of ubiquitin, SUMO2/3, and ISG15 conjugates with MFN1/2 is 70 kD and above when considering the molecular weight of MFN1/2 at 85 kD. g Quantification of MFN1/2, ubiquitin, SUMO2/3, and ISG15 of (f) using ImageJ after immunoprecipitation of MFN1/2. n = 3 per group. h IB analysis of MFN1/2, MFN1, MFN2, ubiquitin, SUMO2/3, and ISG15 in PHCs following either knockdown or overexpression of PARP12 after immunoprecipitation of MFN1/2. i Quantification of MFN1/2, ubiquitin, SUMO2/3, and ISG15 of (h) using ImageJ. n = 3 per group. j MitoTracker Red and LysoTracker Green staining of PHCs was observed by confocal microscopy. Scale bars: 10 µm. k Western blot analysis of PARP12, ISG15, LC3B, p62, MFN1, MFN2, PINK1, Parkin, COL2A1, aggrecan, MMP13, RUNX2, Bcl2, Bax, and NLRP3 inflammasome activity in PHCs coinfected with PARP12 KD-03 shRNA and ISG15-OE adenovirus. n = 3 per group. l ROS staining with PARP12 knockdown and ISG15 overexpression. Scale bars: 100 µm. m JC-1 staining in PHCs following transfection with PARP12 knockdown and ISG15 overexpression. Scale bars: 100 µm. Data are presented as the mean ± SD. Paired t-test (b, d, i) and one-way analysis of variance followed by Tukey’s multiple comparison test (g) were used for statistical analysis. *P < 0.05, **P < 0.01, and ***P < 0.001
Sumoylation 2 3 Affinity Beads, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/signal+detection/pm39465252-284-6-11?v=Cytoskeleton+Inc
Average 93 stars, based on 1 article reviews
sumoylation 2 3 affinity beads - by Bioz Stars, 2026-07
93/100 stars
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bk161 cytoskeleton  (Cytoskeleton Inc)
94
Cytoskeleton Inc bk161 cytoskeleton
Fig. 5 ISG15 upregulated by PAPR12, increasing ISGylation of MFN1/2 and then attenuating the ubiquitylation and <t>SUMOylation</t> to inhibit PINK1/Parkin-dependent mitophagy. a Immunoblotting (IB) analysis of ubiquitylation and ISG15 in PHCs following transfection with KD-ISG15 or KD-NC. b Protein quantification of (a) using ImageJ. n = 3 per group. c IB analysis of ubiquitylation of mitochondrial proteins. Mitochondria were isolated from KD-NC and KD-ISG15 PHCs transfected with a HA-ubiquitin construct. d Protein quantification of (d) using ImageJ. n = 3 per group. e IB analysis of SUMO2/3 and ubiquitin with SUMO2/3-conjugated proteins in KD-NC and KD-ISG15 PHCs immunoprecipitated using SUMO2/3 affinity beads. f IB analysis of MFN1/2, MFN1, MFN2, ubiquitin, SUMO2/3 and ISG15 in KD-NC and KD-ISG15 PHCs treated with or without MG-132 after immunoprecipitation of MFN1/2. The molecular marker of IB plots of ubiquitin, SUMO2/3, and ISG15 conjugates with MFN1/2 is 70 kD and above when considering the molecular weight of MFN1/2 at 85 kD. g Quantification of MFN1/2, ubiquitin, SUMO2/3, and ISG15 of (f) using ImageJ after immunoprecipitation of MFN1/2. n = 3 per group. h IB analysis of MFN1/2, MFN1, MFN2, ubiquitin, SUMO2/3, and ISG15 in PHCs following either knockdown or overexpression of PARP12 after immunoprecipitation of MFN1/2. i Quantification of MFN1/2, ubiquitin, SUMO2/3, and ISG15 of (h) using ImageJ. n = 3 per group. j MitoTracker Red and LysoTracker Green staining of PHCs was observed by confocal microscopy. Scale bars: 10 µm. k Western blot analysis of PARP12, ISG15, LC3B, p62, MFN1, MFN2, PINK1, Parkin, COL2A1, aggrecan, MMP13, RUNX2, Bcl2, Bax, and NLRP3 inflammasome activity in PHCs coinfected with PARP12 KD-03 shRNA and ISG15-OE adenovirus. n = 3 per group. l ROS staining with PARP12 knockdown and ISG15 overexpression. Scale bars: 100 µm. m JC-1 staining in PHCs following transfection with PARP12 knockdown and ISG15 overexpression. Scale bars: 100 µm. Data are presented as the mean ± SD. Paired t-test (b, d, i) and one-way analysis of variance followed by Tukey’s multiple comparison test (g) were used for statistical analysis. *P < 0.05, **P < 0.01, and ***P < 0.001
Bk161 Cytoskeleton, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/signal+detection/pm40215271-359-11-12?v=Cytoskeleton+Inc
Average 94 stars, based on 1 article reviews
bk161 cytoskeleton - by Bioz Stars, 2026-07
94/100 stars
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signal seeker acetyl lysine detection kit  (Cytoskeleton Inc)
94
Cytoskeleton Inc signal seeker acetyl lysine detection kit
Fig. 5 ISG15 upregulated by PAPR12, increasing ISGylation of MFN1/2 and then attenuating the ubiquitylation and <t>SUMOylation</t> to inhibit PINK1/Parkin-dependent mitophagy. a Immunoblotting (IB) analysis of ubiquitylation and ISG15 in PHCs following transfection with KD-ISG15 or KD-NC. b Protein quantification of (a) using ImageJ. n = 3 per group. c IB analysis of ubiquitylation of mitochondrial proteins. Mitochondria were isolated from KD-NC and KD-ISG15 PHCs transfected with a HA-ubiquitin construct. d Protein quantification of (d) using ImageJ. n = 3 per group. e IB analysis of SUMO2/3 and ubiquitin with SUMO2/3-conjugated proteins in KD-NC and KD-ISG15 PHCs immunoprecipitated using SUMO2/3 affinity beads. f IB analysis of MFN1/2, MFN1, MFN2, ubiquitin, SUMO2/3 and ISG15 in KD-NC and KD-ISG15 PHCs treated with or without MG-132 after immunoprecipitation of MFN1/2. The molecular marker of IB plots of ubiquitin, SUMO2/3, and ISG15 conjugates with MFN1/2 is 70 kD and above when considering the molecular weight of MFN1/2 at 85 kD. g Quantification of MFN1/2, ubiquitin, SUMO2/3, and ISG15 of (f) using ImageJ after immunoprecipitation of MFN1/2. n = 3 per group. h IB analysis of MFN1/2, MFN1, MFN2, ubiquitin, SUMO2/3, and ISG15 in PHCs following either knockdown or overexpression of PARP12 after immunoprecipitation of MFN1/2. i Quantification of MFN1/2, ubiquitin, SUMO2/3, and ISG15 of (h) using ImageJ. n = 3 per group. j MitoTracker Red and LysoTracker Green staining of PHCs was observed by confocal microscopy. Scale bars: 10 µm. k Western blot analysis of PARP12, ISG15, LC3B, p62, MFN1, MFN2, PINK1, Parkin, COL2A1, aggrecan, MMP13, RUNX2, Bcl2, Bax, and NLRP3 inflammasome activity in PHCs coinfected with PARP12 KD-03 shRNA and ISG15-OE adenovirus. n = 3 per group. l ROS staining with PARP12 knockdown and ISG15 overexpression. Scale bars: 100 µm. m JC-1 staining in PHCs following transfection with PARP12 knockdown and ISG15 overexpression. Scale bars: 100 µm. Data are presented as the mean ± SD. Paired t-test (b, d, i) and one-way analysis of variance followed by Tukey’s multiple comparison test (g) were used for statistical analysis. *P < 0.05, **P < 0.01, and ***P < 0.001
Signal Seeker Acetyl Lysine Detection Kit, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/signal+detection/pm39769333-349-8-14?v=Cytoskeleton+Inc
Average 94 stars, based on 1 article reviews
signal seeker acetyl lysine detection kit - by Bioz Stars, 2026-07
94/100 stars
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signal seeker sumoylation 1  (Cytoskeleton Inc)
93
Cytoskeleton Inc signal seeker sumoylation 1
Fig. 5 ISG15 upregulated by PAPR12, increasing ISGylation of MFN1/2 and then attenuating the ubiquitylation and <t>SUMOylation</t> to inhibit PINK1/Parkin-dependent mitophagy. a Immunoblotting (IB) analysis of ubiquitylation and ISG15 in PHCs following transfection with KD-ISG15 or KD-NC. b Protein quantification of (a) using ImageJ. n = 3 per group. c IB analysis of ubiquitylation of mitochondrial proteins. Mitochondria were isolated from KD-NC and KD-ISG15 PHCs transfected with a HA-ubiquitin construct. d Protein quantification of (d) using ImageJ. n = 3 per group. e IB analysis of SUMO2/3 and ubiquitin with SUMO2/3-conjugated proteins in KD-NC and KD-ISG15 PHCs immunoprecipitated using SUMO2/3 affinity beads. f IB analysis of MFN1/2, MFN1, MFN2, ubiquitin, SUMO2/3 and ISG15 in KD-NC and KD-ISG15 PHCs treated with or without MG-132 after immunoprecipitation of MFN1/2. The molecular marker of IB plots of ubiquitin, SUMO2/3, and ISG15 conjugates with MFN1/2 is 70 kD and above when considering the molecular weight of MFN1/2 at 85 kD. g Quantification of MFN1/2, ubiquitin, SUMO2/3, and ISG15 of (f) using ImageJ after immunoprecipitation of MFN1/2. n = 3 per group. h IB analysis of MFN1/2, MFN1, MFN2, ubiquitin, SUMO2/3, and ISG15 in PHCs following either knockdown or overexpression of PARP12 after immunoprecipitation of MFN1/2. i Quantification of MFN1/2, ubiquitin, SUMO2/3, and ISG15 of (h) using ImageJ. n = 3 per group. j MitoTracker Red and LysoTracker Green staining of PHCs was observed by confocal microscopy. Scale bars: 10 µm. k Western blot analysis of PARP12, ISG15, LC3B, p62, MFN1, MFN2, PINK1, Parkin, COL2A1, aggrecan, MMP13, RUNX2, Bcl2, Bax, and NLRP3 inflammasome activity in PHCs coinfected with PARP12 KD-03 shRNA and ISG15-OE adenovirus. n = 3 per group. l ROS staining with PARP12 knockdown and ISG15 overexpression. Scale bars: 100 µm. m JC-1 staining in PHCs following transfection with PARP12 knockdown and ISG15 overexpression. Scale bars: 100 µm. Data are presented as the mean ± SD. Paired t-test (b, d, i) and one-way analysis of variance followed by Tukey’s multiple comparison test (g) were used for statistical analysis. *P < 0.05, **P < 0.01, and ***P < 0.001
Signal Seeker Sumoylation 1, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/signal+detection/bio_rxiv__2024__08__22__609092-314-28-36?v=Cytoskeleton+Inc
Average 93 stars, based on 1 article reviews
signal seeker sumoylation 1 - by Bioz Stars, 2026-07
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chemoluminescence signal detection immobilon  (Merck KGaA)
90
Merck KGaA chemoluminescence signal detection immobilon
Fig. 5 ISG15 upregulated by PAPR12, increasing ISGylation of MFN1/2 and then attenuating the ubiquitylation and <t>SUMOylation</t> to inhibit PINK1/Parkin-dependent mitophagy. a Immunoblotting (IB) analysis of ubiquitylation and ISG15 in PHCs following transfection with KD-ISG15 or KD-NC. b Protein quantification of (a) using ImageJ. n = 3 per group. c IB analysis of ubiquitylation of mitochondrial proteins. Mitochondria were isolated from KD-NC and KD-ISG15 PHCs transfected with a HA-ubiquitin construct. d Protein quantification of (d) using ImageJ. n = 3 per group. e IB analysis of SUMO2/3 and ubiquitin with SUMO2/3-conjugated proteins in KD-NC and KD-ISG15 PHCs immunoprecipitated using SUMO2/3 affinity beads. f IB analysis of MFN1/2, MFN1, MFN2, ubiquitin, SUMO2/3 and ISG15 in KD-NC and KD-ISG15 PHCs treated with or without MG-132 after immunoprecipitation of MFN1/2. The molecular marker of IB plots of ubiquitin, SUMO2/3, and ISG15 conjugates with MFN1/2 is 70 kD and above when considering the molecular weight of MFN1/2 at 85 kD. g Quantification of MFN1/2, ubiquitin, SUMO2/3, and ISG15 of (f) using ImageJ after immunoprecipitation of MFN1/2. n = 3 per group. h IB analysis of MFN1/2, MFN1, MFN2, ubiquitin, SUMO2/3, and ISG15 in PHCs following either knockdown or overexpression of PARP12 after immunoprecipitation of MFN1/2. i Quantification of MFN1/2, ubiquitin, SUMO2/3, and ISG15 of (h) using ImageJ. n = 3 per group. j MitoTracker Red and LysoTracker Green staining of PHCs was observed by confocal microscopy. Scale bars: 10 µm. k Western blot analysis of PARP12, ISG15, LC3B, p62, MFN1, MFN2, PINK1, Parkin, COL2A1, aggrecan, MMP13, RUNX2, Bcl2, Bax, and NLRP3 inflammasome activity in PHCs coinfected with PARP12 KD-03 shRNA and ISG15-OE adenovirus. n = 3 per group. l ROS staining with PARP12 knockdown and ISG15 overexpression. Scale bars: 100 µm. m JC-1 staining in PHCs following transfection with PARP12 knockdown and ISG15 overexpression. Scale bars: 100 µm. Data are presented as the mean ± SD. Paired t-test (b, d, i) and one-way analysis of variance followed by Tukey’s multiple comparison test (g) were used for statistical analysis. *P < 0.05, **P < 0.01, and ***P < 0.001
Chemoluminescence Signal Detection Immobilon, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/signal+detection/pm30226610-57-10-14?v=Merck+KGaA
Average 90 stars, based on 1 article reviews
chemoluminescence signal detection immobilon - by Bioz Stars, 2026-07
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nanoluciferase signal detection system  (Promega)
90
Promega nanoluciferase signal detection system
Fig. 5 ISG15 upregulated by PAPR12, increasing ISGylation of MFN1/2 and then attenuating the ubiquitylation and <t>SUMOylation</t> to inhibit PINK1/Parkin-dependent mitophagy. a Immunoblotting (IB) analysis of ubiquitylation and ISG15 in PHCs following transfection with KD-ISG15 or KD-NC. b Protein quantification of (a) using ImageJ. n = 3 per group. c IB analysis of ubiquitylation of mitochondrial proteins. Mitochondria were isolated from KD-NC and KD-ISG15 PHCs transfected with a HA-ubiquitin construct. d Protein quantification of (d) using ImageJ. n = 3 per group. e IB analysis of SUMO2/3 and ubiquitin with SUMO2/3-conjugated proteins in KD-NC and KD-ISG15 PHCs immunoprecipitated using SUMO2/3 affinity beads. f IB analysis of MFN1/2, MFN1, MFN2, ubiquitin, SUMO2/3 and ISG15 in KD-NC and KD-ISG15 PHCs treated with or without MG-132 after immunoprecipitation of MFN1/2. The molecular marker of IB plots of ubiquitin, SUMO2/3, and ISG15 conjugates with MFN1/2 is 70 kD and above when considering the molecular weight of MFN1/2 at 85 kD. g Quantification of MFN1/2, ubiquitin, SUMO2/3, and ISG15 of (f) using ImageJ after immunoprecipitation of MFN1/2. n = 3 per group. h IB analysis of MFN1/2, MFN1, MFN2, ubiquitin, SUMO2/3, and ISG15 in PHCs following either knockdown or overexpression of PARP12 after immunoprecipitation of MFN1/2. i Quantification of MFN1/2, ubiquitin, SUMO2/3, and ISG15 of (h) using ImageJ. n = 3 per group. j MitoTracker Red and LysoTracker Green staining of PHCs was observed by confocal microscopy. Scale bars: 10 µm. k Western blot analysis of PARP12, ISG15, LC3B, p62, MFN1, MFN2, PINK1, Parkin, COL2A1, aggrecan, MMP13, RUNX2, Bcl2, Bax, and NLRP3 inflammasome activity in PHCs coinfected with PARP12 KD-03 shRNA and ISG15-OE adenovirus. n = 3 per group. l ROS staining with PARP12 knockdown and ISG15 overexpression. Scale bars: 100 µm. m JC-1 staining in PHCs following transfection with PARP12 knockdown and ISG15 overexpression. Scale bars: 100 µm. Data are presented as the mean ± SD. Paired t-test (b, d, i) and one-way analysis of variance followed by Tukey’s multiple comparison test (g) were used for statistical analysis. *P < 0.05, **P < 0.01, and ***P < 0.001
Nanoluciferase Signal Detection System, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/signal+detection/pm32980446-84-0-10?v=Promega
Average 90 stars, based on 1 article reviews
nanoluciferase signal detection system - by Bioz Stars, 2026-07
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ultrasound signal detection device  (LimFlow GmbH)
90
LimFlow GmbH ultrasound signal detection device
Fig. 5 ISG15 upregulated by PAPR12, increasing ISGylation of MFN1/2 and then attenuating the ubiquitylation and <t>SUMOylation</t> to inhibit PINK1/Parkin-dependent mitophagy. a Immunoblotting (IB) analysis of ubiquitylation and ISG15 in PHCs following transfection with KD-ISG15 or KD-NC. b Protein quantification of (a) using ImageJ. n = 3 per group. c IB analysis of ubiquitylation of mitochondrial proteins. Mitochondria were isolated from KD-NC and KD-ISG15 PHCs transfected with a HA-ubiquitin construct. d Protein quantification of (d) using ImageJ. n = 3 per group. e IB analysis of SUMO2/3 and ubiquitin with SUMO2/3-conjugated proteins in KD-NC and KD-ISG15 PHCs immunoprecipitated using SUMO2/3 affinity beads. f IB analysis of MFN1/2, MFN1, MFN2, ubiquitin, SUMO2/3 and ISG15 in KD-NC and KD-ISG15 PHCs treated with or without MG-132 after immunoprecipitation of MFN1/2. The molecular marker of IB plots of ubiquitin, SUMO2/3, and ISG15 conjugates with MFN1/2 is 70 kD and above when considering the molecular weight of MFN1/2 at 85 kD. g Quantification of MFN1/2, ubiquitin, SUMO2/3, and ISG15 of (f) using ImageJ after immunoprecipitation of MFN1/2. n = 3 per group. h IB analysis of MFN1/2, MFN1, MFN2, ubiquitin, SUMO2/3, and ISG15 in PHCs following either knockdown or overexpression of PARP12 after immunoprecipitation of MFN1/2. i Quantification of MFN1/2, ubiquitin, SUMO2/3, and ISG15 of (h) using ImageJ. n = 3 per group. j MitoTracker Red and LysoTracker Green staining of PHCs was observed by confocal microscopy. Scale bars: 10 µm. k Western blot analysis of PARP12, ISG15, LC3B, p62, MFN1, MFN2, PINK1, Parkin, COL2A1, aggrecan, MMP13, RUNX2, Bcl2, Bax, and NLRP3 inflammasome activity in PHCs coinfected with PARP12 KD-03 shRNA and ISG15-OE adenovirus. n = 3 per group. l ROS staining with PARP12 knockdown and ISG15 overexpression. Scale bars: 100 µm. m JC-1 staining in PHCs following transfection with PARP12 knockdown and ISG15 overexpression. Scale bars: 100 µm. Data are presented as the mean ± SD. Paired t-test (b, d, i) and one-way analysis of variance followed by Tukey’s multiple comparison test (g) were used for statistical analysis. *P < 0.05, **P < 0.01, and ***P < 0.001
Ultrasound Signal Detection Device, supplied by LimFlow GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/signal+detection/pmc08261698-105-6-2?v=LimFlow+GmbH
Average 90 stars, based on 1 article reviews
ultrasound signal detection device - by Bioz Stars, 2026-07
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optical detection signal with a λ1 wavelength  (Station X)
90
Station X optical detection signal with a λ1 wavelength
Fig. 5 ISG15 upregulated by PAPR12, increasing ISGylation of MFN1/2 and then attenuating the ubiquitylation and <t>SUMOylation</t> to inhibit PINK1/Parkin-dependent mitophagy. a Immunoblotting (IB) analysis of ubiquitylation and ISG15 in PHCs following transfection with KD-ISG15 or KD-NC. b Protein quantification of (a) using ImageJ. n = 3 per group. c IB analysis of ubiquitylation of mitochondrial proteins. Mitochondria were isolated from KD-NC and KD-ISG15 PHCs transfected with a HA-ubiquitin construct. d Protein quantification of (d) using ImageJ. n = 3 per group. e IB analysis of SUMO2/3 and ubiquitin with SUMO2/3-conjugated proteins in KD-NC and KD-ISG15 PHCs immunoprecipitated using SUMO2/3 affinity beads. f IB analysis of MFN1/2, MFN1, MFN2, ubiquitin, SUMO2/3 and ISG15 in KD-NC and KD-ISG15 PHCs treated with or without MG-132 after immunoprecipitation of MFN1/2. The molecular marker of IB plots of ubiquitin, SUMO2/3, and ISG15 conjugates with MFN1/2 is 70 kD and above when considering the molecular weight of MFN1/2 at 85 kD. g Quantification of MFN1/2, ubiquitin, SUMO2/3, and ISG15 of (f) using ImageJ after immunoprecipitation of MFN1/2. n = 3 per group. h IB analysis of MFN1/2, MFN1, MFN2, ubiquitin, SUMO2/3, and ISG15 in PHCs following either knockdown or overexpression of PARP12 after immunoprecipitation of MFN1/2. i Quantification of MFN1/2, ubiquitin, SUMO2/3, and ISG15 of (h) using ImageJ. n = 3 per group. j MitoTracker Red and LysoTracker Green staining of PHCs was observed by confocal microscopy. Scale bars: 10 µm. k Western blot analysis of PARP12, ISG15, LC3B, p62, MFN1, MFN2, PINK1, Parkin, COL2A1, aggrecan, MMP13, RUNX2, Bcl2, Bax, and NLRP3 inflammasome activity in PHCs coinfected with PARP12 KD-03 shRNA and ISG15-OE adenovirus. n = 3 per group. l ROS staining with PARP12 knockdown and ISG15 overexpression. Scale bars: 100 µm. m JC-1 staining in PHCs following transfection with PARP12 knockdown and ISG15 overexpression. Scale bars: 100 µm. Data are presented as the mean ± SD. Paired t-test (b, d, i) and one-way analysis of variance followed by Tukey’s multiple comparison test (g) were used for statistical analysis. *P < 0.05, **P < 0.01, and ***P < 0.001
Optical Detection Signal With A λ1 Wavelength, supplied by Station X, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/signal+detection/us09059797-182-14-5?v=Station+X
Average 90 stars, based on 1 article reviews
optical detection signal with a λ1 wavelength - by Bioz Stars, 2026-07
90/100 stars
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luminescent signal detection chamber  (Vilber Lourmat)
90
Vilber Lourmat luminescent signal detection chamber
Fig. 5 ISG15 upregulated by PAPR12, increasing ISGylation of MFN1/2 and then attenuating the ubiquitylation and <t>SUMOylation</t> to inhibit PINK1/Parkin-dependent mitophagy. a Immunoblotting (IB) analysis of ubiquitylation and ISG15 in PHCs following transfection with KD-ISG15 or KD-NC. b Protein quantification of (a) using ImageJ. n = 3 per group. c IB analysis of ubiquitylation of mitochondrial proteins. Mitochondria were isolated from KD-NC and KD-ISG15 PHCs transfected with a HA-ubiquitin construct. d Protein quantification of (d) using ImageJ. n = 3 per group. e IB analysis of SUMO2/3 and ubiquitin with SUMO2/3-conjugated proteins in KD-NC and KD-ISG15 PHCs immunoprecipitated using SUMO2/3 affinity beads. f IB analysis of MFN1/2, MFN1, MFN2, ubiquitin, SUMO2/3 and ISG15 in KD-NC and KD-ISG15 PHCs treated with or without MG-132 after immunoprecipitation of MFN1/2. The molecular marker of IB plots of ubiquitin, SUMO2/3, and ISG15 conjugates with MFN1/2 is 70 kD and above when considering the molecular weight of MFN1/2 at 85 kD. g Quantification of MFN1/2, ubiquitin, SUMO2/3, and ISG15 of (f) using ImageJ after immunoprecipitation of MFN1/2. n = 3 per group. h IB analysis of MFN1/2, MFN1, MFN2, ubiquitin, SUMO2/3, and ISG15 in PHCs following either knockdown or overexpression of PARP12 after immunoprecipitation of MFN1/2. i Quantification of MFN1/2, ubiquitin, SUMO2/3, and ISG15 of (h) using ImageJ. n = 3 per group. j MitoTracker Red and LysoTracker Green staining of PHCs was observed by confocal microscopy. Scale bars: 10 µm. k Western blot analysis of PARP12, ISG15, LC3B, p62, MFN1, MFN2, PINK1, Parkin, COL2A1, aggrecan, MMP13, RUNX2, Bcl2, Bax, and NLRP3 inflammasome activity in PHCs coinfected with PARP12 KD-03 shRNA and ISG15-OE adenovirus. n = 3 per group. l ROS staining with PARP12 knockdown and ISG15 overexpression. Scale bars: 100 µm. m JC-1 staining in PHCs following transfection with PARP12 knockdown and ISG15 overexpression. Scale bars: 100 µm. Data are presented as the mean ± SD. Paired t-test (b, d, i) and one-way analysis of variance followed by Tukey’s multiple comparison test (g) were used for statistical analysis. *P < 0.05, **P < 0.01, and ***P < 0.001
Luminescent Signal Detection Chamber, supplied by Vilber Lourmat, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/signal+detection/pm37277577-69-1-16?v=Vilber+Lourmat
Average 90 stars, based on 1 article reviews
luminescent signal detection chamber - by Bioz Stars, 2026-07
90/100 stars
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signal detection in non-gaussian noise  (Verlag GmbH)
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Verlag GmbH signal detection in non-gaussian noise
Fig. 5 ISG15 upregulated by PAPR12, increasing ISGylation of MFN1/2 and then attenuating the ubiquitylation and <t>SUMOylation</t> to inhibit PINK1/Parkin-dependent mitophagy. a Immunoblotting (IB) analysis of ubiquitylation and ISG15 in PHCs following transfection with KD-ISG15 or KD-NC. b Protein quantification of (a) using ImageJ. n = 3 per group. c IB analysis of ubiquitylation of mitochondrial proteins. Mitochondria were isolated from KD-NC and KD-ISG15 PHCs transfected with a HA-ubiquitin construct. d Protein quantification of (d) using ImageJ. n = 3 per group. e IB analysis of SUMO2/3 and ubiquitin with SUMO2/3-conjugated proteins in KD-NC and KD-ISG15 PHCs immunoprecipitated using SUMO2/3 affinity beads. f IB analysis of MFN1/2, MFN1, MFN2, ubiquitin, SUMO2/3 and ISG15 in KD-NC and KD-ISG15 PHCs treated with or without MG-132 after immunoprecipitation of MFN1/2. The molecular marker of IB plots of ubiquitin, SUMO2/3, and ISG15 conjugates with MFN1/2 is 70 kD and above when considering the molecular weight of MFN1/2 at 85 kD. g Quantification of MFN1/2, ubiquitin, SUMO2/3, and ISG15 of (f) using ImageJ after immunoprecipitation of MFN1/2. n = 3 per group. h IB analysis of MFN1/2, MFN1, MFN2, ubiquitin, SUMO2/3, and ISG15 in PHCs following either knockdown or overexpression of PARP12 after immunoprecipitation of MFN1/2. i Quantification of MFN1/2, ubiquitin, SUMO2/3, and ISG15 of (h) using ImageJ. n = 3 per group. j MitoTracker Red and LysoTracker Green staining of PHCs was observed by confocal microscopy. Scale bars: 10 µm. k Western blot analysis of PARP12, ISG15, LC3B, p62, MFN1, MFN2, PINK1, Parkin, COL2A1, aggrecan, MMP13, RUNX2, Bcl2, Bax, and NLRP3 inflammasome activity in PHCs coinfected with PARP12 KD-03 shRNA and ISG15-OE adenovirus. n = 3 per group. l ROS staining with PARP12 knockdown and ISG15 overexpression. Scale bars: 100 µm. m JC-1 staining in PHCs following transfection with PARP12 knockdown and ISG15 overexpression. Scale bars: 100 µm. Data are presented as the mean ± SD. Paired t-test (b, d, i) and one-way analysis of variance followed by Tukey’s multiple comparison test (g) were used for statistical analysis. *P < 0.05, **P < 0.01, and ***P < 0.001
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Fig. 5 ISG15 upregulated by PAPR12, increasing ISGylation of MFN1/2 and then attenuating the ubiquitylation and <t>SUMOylation</t> to inhibit PINK1/Parkin-dependent mitophagy. a Immunoblotting (IB) analysis of ubiquitylation and ISG15 in PHCs following transfection with KD-ISG15 or KD-NC. b Protein quantification of (a) using ImageJ. n = 3 per group. c IB analysis of ubiquitylation of mitochondrial proteins. Mitochondria were isolated from KD-NC and KD-ISG15 PHCs transfected with a HA-ubiquitin construct. d Protein quantification of (d) using ImageJ. n = 3 per group. e IB analysis of SUMO2/3 and ubiquitin with SUMO2/3-conjugated proteins in KD-NC and KD-ISG15 PHCs immunoprecipitated using SUMO2/3 affinity beads. f IB analysis of MFN1/2, MFN1, MFN2, ubiquitin, SUMO2/3 and ISG15 in KD-NC and KD-ISG15 PHCs treated with or without MG-132 after immunoprecipitation of MFN1/2. The molecular marker of IB plots of ubiquitin, SUMO2/3, and ISG15 conjugates with MFN1/2 is 70 kD and above when considering the molecular weight of MFN1/2 at 85 kD. g Quantification of MFN1/2, ubiquitin, SUMO2/3, and ISG15 of (f) using ImageJ after immunoprecipitation of MFN1/2. n = 3 per group. h IB analysis of MFN1/2, MFN1, MFN2, ubiquitin, SUMO2/3, and ISG15 in PHCs following either knockdown or overexpression of PARP12 after immunoprecipitation of MFN1/2. i Quantification of MFN1/2, ubiquitin, SUMO2/3, and ISG15 of (h) using ImageJ. n = 3 per group. j MitoTracker Red and LysoTracker Green staining of PHCs was observed by confocal microscopy. Scale bars: 10 µm. k Western blot analysis of PARP12, ISG15, LC3B, p62, MFN1, MFN2, PINK1, Parkin, COL2A1, aggrecan, MMP13, RUNX2, Bcl2, Bax, and NLRP3 inflammasome activity in PHCs coinfected with PARP12 KD-03 shRNA and ISG15-OE adenovirus. n = 3 per group. l ROS staining with PARP12 knockdown and ISG15 overexpression. Scale bars: 100 µm. m JC-1 staining in PHCs following transfection with PARP12 knockdown and ISG15 overexpression. Scale bars: 100 µm. Data are presented as the mean ± SD. Paired t-test (b, d, i) and one-way analysis of variance followed by Tukey’s multiple comparison test (g) were used for statistical analysis. *P < 0.05, **P < 0.01, and ***P < 0.001
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Fig. 5 ISG15 upregulated by PAPR12, increasing ISGylation of MFN1/2 and then attenuating the ubiquitylation and <t>SUMOylation</t> to inhibit PINK1/Parkin-dependent mitophagy. a Immunoblotting (IB) analysis of ubiquitylation and ISG15 in PHCs following transfection with KD-ISG15 or KD-NC. b Protein quantification of (a) using ImageJ. n = 3 per group. c IB analysis of ubiquitylation of mitochondrial proteins. Mitochondria were isolated from KD-NC and KD-ISG15 PHCs transfected with a HA-ubiquitin construct. d Protein quantification of (d) using ImageJ. n = 3 per group. e IB analysis of SUMO2/3 and ubiquitin with SUMO2/3-conjugated proteins in KD-NC and KD-ISG15 PHCs immunoprecipitated using SUMO2/3 affinity beads. f IB analysis of MFN1/2, MFN1, MFN2, ubiquitin, SUMO2/3 and ISG15 in KD-NC and KD-ISG15 PHCs treated with or without MG-132 after immunoprecipitation of MFN1/2. The molecular marker of IB plots of ubiquitin, SUMO2/3, and ISG15 conjugates with MFN1/2 is 70 kD and above when considering the molecular weight of MFN1/2 at 85 kD. g Quantification of MFN1/2, ubiquitin, SUMO2/3, and ISG15 of (f) using ImageJ after immunoprecipitation of MFN1/2. n = 3 per group. h IB analysis of MFN1/2, MFN1, MFN2, ubiquitin, SUMO2/3, and ISG15 in PHCs following either knockdown or overexpression of PARP12 after immunoprecipitation of MFN1/2. i Quantification of MFN1/2, ubiquitin, SUMO2/3, and ISG15 of (h) using ImageJ. n = 3 per group. j MitoTracker Red and LysoTracker Green staining of PHCs was observed by confocal microscopy. Scale bars: 10 µm. k Western blot analysis of PARP12, ISG15, LC3B, p62, MFN1, MFN2, PINK1, Parkin, COL2A1, aggrecan, MMP13, RUNX2, Bcl2, Bax, and NLRP3 inflammasome activity in PHCs coinfected with PARP12 KD-03 shRNA and ISG15-OE adenovirus. n = 3 per group. l ROS staining with PARP12 knockdown and ISG15 overexpression. Scale bars: 100 µm. m JC-1 staining in PHCs following transfection with PARP12 knockdown and ISG15 overexpression. Scale bars: 100 µm. Data are presented as the mean ± SD. Paired t-test (b, d, i) and one-way analysis of variance followed by Tukey’s multiple comparison test (g) were used for statistical analysis. *P < 0.05, **P < 0.01, and ***P < 0.001
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Image Search Results


Fig. 5 ISG15 upregulated by PAPR12, increasing ISGylation of MFN1/2 and then attenuating the ubiquitylation and SUMOylation to inhibit PINK1/Parkin-dependent mitophagy. a Immunoblotting (IB) analysis of ubiquitylation and ISG15 in PHCs following transfection with KD-ISG15 or KD-NC. b Protein quantification of (a) using ImageJ. n = 3 per group. c IB analysis of ubiquitylation of mitochondrial proteins. Mitochondria were isolated from KD-NC and KD-ISG15 PHCs transfected with a HA-ubiquitin construct. d Protein quantification of (d) using ImageJ. n = 3 per group. e IB analysis of SUMO2/3 and ubiquitin with SUMO2/3-conjugated proteins in KD-NC and KD-ISG15 PHCs immunoprecipitated using SUMO2/3 affinity beads. f IB analysis of MFN1/2, MFN1, MFN2, ubiquitin, SUMO2/3 and ISG15 in KD-NC and KD-ISG15 PHCs treated with or without MG-132 after immunoprecipitation of MFN1/2. The molecular marker of IB plots of ubiquitin, SUMO2/3, and ISG15 conjugates with MFN1/2 is 70 kD and above when considering the molecular weight of MFN1/2 at 85 kD. g Quantification of MFN1/2, ubiquitin, SUMO2/3, and ISG15 of (f) using ImageJ after immunoprecipitation of MFN1/2. n = 3 per group. h IB analysis of MFN1/2, MFN1, MFN2, ubiquitin, SUMO2/3, and ISG15 in PHCs following either knockdown or overexpression of PARP12 after immunoprecipitation of MFN1/2. i Quantification of MFN1/2, ubiquitin, SUMO2/3, and ISG15 of (h) using ImageJ. n = 3 per group. j MitoTracker Red and LysoTracker Green staining of PHCs was observed by confocal microscopy. Scale bars: 10 µm. k Western blot analysis of PARP12, ISG15, LC3B, p62, MFN1, MFN2, PINK1, Parkin, COL2A1, aggrecan, MMP13, RUNX2, Bcl2, Bax, and NLRP3 inflammasome activity in PHCs coinfected with PARP12 KD-03 shRNA and ISG15-OE adenovirus. n = 3 per group. l ROS staining with PARP12 knockdown and ISG15 overexpression. Scale bars: 100 µm. m JC-1 staining in PHCs following transfection with PARP12 knockdown and ISG15 overexpression. Scale bars: 100 µm. Data are presented as the mean ± SD. Paired t-test (b, d, i) and one-way analysis of variance followed by Tukey’s multiple comparison test (g) were used for statistical analysis. *P < 0.05, **P < 0.01, and ***P < 0.001

Journal: Bone research

Article Title: IRF1-mediated upregulation of PARP12 promotes cartilage degradation by inhibiting PINK1/Parkin dependent mitophagy through ISG15 attenuating ubiquitylation and SUMOylation of MFN1/2.

doi: 10.1038/s41413-024-00363-3

Figure Lengend Snippet: Fig. 5 ISG15 upregulated by PAPR12, increasing ISGylation of MFN1/2 and then attenuating the ubiquitylation and SUMOylation to inhibit PINK1/Parkin-dependent mitophagy. a Immunoblotting (IB) analysis of ubiquitylation and ISG15 in PHCs following transfection with KD-ISG15 or KD-NC. b Protein quantification of (a) using ImageJ. n = 3 per group. c IB analysis of ubiquitylation of mitochondrial proteins. Mitochondria were isolated from KD-NC and KD-ISG15 PHCs transfected with a HA-ubiquitin construct. d Protein quantification of (d) using ImageJ. n = 3 per group. e IB analysis of SUMO2/3 and ubiquitin with SUMO2/3-conjugated proteins in KD-NC and KD-ISG15 PHCs immunoprecipitated using SUMO2/3 affinity beads. f IB analysis of MFN1/2, MFN1, MFN2, ubiquitin, SUMO2/3 and ISG15 in KD-NC and KD-ISG15 PHCs treated with or without MG-132 after immunoprecipitation of MFN1/2. The molecular marker of IB plots of ubiquitin, SUMO2/3, and ISG15 conjugates with MFN1/2 is 70 kD and above when considering the molecular weight of MFN1/2 at 85 kD. g Quantification of MFN1/2, ubiquitin, SUMO2/3, and ISG15 of (f) using ImageJ after immunoprecipitation of MFN1/2. n = 3 per group. h IB analysis of MFN1/2, MFN1, MFN2, ubiquitin, SUMO2/3, and ISG15 in PHCs following either knockdown or overexpression of PARP12 after immunoprecipitation of MFN1/2. i Quantification of MFN1/2, ubiquitin, SUMO2/3, and ISG15 of (h) using ImageJ. n = 3 per group. j MitoTracker Red and LysoTracker Green staining of PHCs was observed by confocal microscopy. Scale bars: 10 µm. k Western blot analysis of PARP12, ISG15, LC3B, p62, MFN1, MFN2, PINK1, Parkin, COL2A1, aggrecan, MMP13, RUNX2, Bcl2, Bax, and NLRP3 inflammasome activity in PHCs coinfected with PARP12 KD-03 shRNA and ISG15-OE adenovirus. n = 3 per group. l ROS staining with PARP12 knockdown and ISG15 overexpression. Scale bars: 100 µm. m JC-1 staining in PHCs following transfection with PARP12 knockdown and ISG15 overexpression. Scale bars: 100 µm. Data are presented as the mean ± SD. Paired t-test (b, d, i) and one-way analysis of variance followed by Tukey’s multiple comparison test (g) were used for statistical analysis. *P < 0.05, **P < 0.01, and ***P < 0.001

Article Snippet: According to the manufacturer’s instructions of SUMOylation 2/3 affinity beads (BK162, Cytoskeleton, Inc.), SUMO2/3-conjugated proteins in chondrocytes lysates were immunoprecipitated.

Techniques: Western Blot, Transfection, Isolation, Ubiquitin Proteomics, Construct, Immunoprecipitation, Marker, Molecular Weight, Knockdown, Over Expression, Staining, Confocal Microscopy, Activity Assay, shRNA, Comparison

Fig. 8 PARP12 modulates osteoarthritis (OA) pathogenesis in monosodium iodoacetate (MIA)-treated rats. a Experimental diagram of the MIA OA rat model treated with XAV-939 or PARP12 overexpression (OE) adenovirus. Rats were evaluated at age of 10 weeks. n = 6 per group. b 3D reconstruction images of micro-CT scanning of the knees of rats treated with XAV-939 or PARP12-OE adenovirus. n = 5 per group. c–f Analysis of BV/TV, BS/TV, trabecular thickness, and trabecular numbers. n = 5 per group. g Western blot analysis of PARP12, COL2A1, aggrecan, MMP13, RUNX2, Bcl2/Bax, LC3B, p62, MFN1, MFN2, PINK1, Parkin and NLRP3 inflammasome activity in chondrocytes of rats treated with XAV-939 or PARP12 overexpression (OE) adenovirus. n = 3 per group. h Representative images of Safranin O and IHC staining of PARP12, COL2A1 and MMP13. Scale bars: 250 µm (first row) and 50 µm (second row). i Quantification of macroscopic score based on staining results in (h). n = 3 per group. j–l Quantification of PARP12, COL2A1, and MMP13 positive chondrocytes based on staining results in (h). n = 3 per group. m–o ROS staining, ATP level and JC-1 staining in chondrocytes of rats treated with XAV-939 or PARP12 OE adenovirus. p Schematic representation of the mechanism by which IRF1-mediated upregulation of PARP12 promotes cartilage degradation by inhibiting PINK1/Parkin dependent mitophagy through ISG15 attenuating the ubiquitylation and SUMOylation of MFN1/2. Data are presented as the mean ± SD. Paired t-test (c–f, j–l, n) and non-parametric Mann-Whitney U test (i) were used for statistical analysis. *P < 0.05, **P < 0.01, ***P < 0.001

Journal: Bone research

Article Title: IRF1-mediated upregulation of PARP12 promotes cartilage degradation by inhibiting PINK1/Parkin dependent mitophagy through ISG15 attenuating ubiquitylation and SUMOylation of MFN1/2.

doi: 10.1038/s41413-024-00363-3

Figure Lengend Snippet: Fig. 8 PARP12 modulates osteoarthritis (OA) pathogenesis in monosodium iodoacetate (MIA)-treated rats. a Experimental diagram of the MIA OA rat model treated with XAV-939 or PARP12 overexpression (OE) adenovirus. Rats were evaluated at age of 10 weeks. n = 6 per group. b 3D reconstruction images of micro-CT scanning of the knees of rats treated with XAV-939 or PARP12-OE adenovirus. n = 5 per group. c–f Analysis of BV/TV, BS/TV, trabecular thickness, and trabecular numbers. n = 5 per group. g Western blot analysis of PARP12, COL2A1, aggrecan, MMP13, RUNX2, Bcl2/Bax, LC3B, p62, MFN1, MFN2, PINK1, Parkin and NLRP3 inflammasome activity in chondrocytes of rats treated with XAV-939 or PARP12 overexpression (OE) adenovirus. n = 3 per group. h Representative images of Safranin O and IHC staining of PARP12, COL2A1 and MMP13. Scale bars: 250 µm (first row) and 50 µm (second row). i Quantification of macroscopic score based on staining results in (h). n = 3 per group. j–l Quantification of PARP12, COL2A1, and MMP13 positive chondrocytes based on staining results in (h). n = 3 per group. m–o ROS staining, ATP level and JC-1 staining in chondrocytes of rats treated with XAV-939 or PARP12 OE adenovirus. p Schematic representation of the mechanism by which IRF1-mediated upregulation of PARP12 promotes cartilage degradation by inhibiting PINK1/Parkin dependent mitophagy through ISG15 attenuating the ubiquitylation and SUMOylation of MFN1/2. Data are presented as the mean ± SD. Paired t-test (c–f, j–l, n) and non-parametric Mann-Whitney U test (i) were used for statistical analysis. *P < 0.05, **P < 0.01, ***P < 0.001

Article Snippet: According to the manufacturer’s instructions of SUMOylation 2/3 affinity beads (BK162, Cytoskeleton, Inc.), SUMO2/3-conjugated proteins in chondrocytes lysates were immunoprecipitated.

Techniques: Over Expression, Micro-CT, Western Blot, Activity Assay, Immunohistochemistry, Staining, MANN-WHITNEY